山羊睾丸和附睾头β防御素家族的表达谱及生物信息学分析

doi:10.11843/j.issn.0366-6964.2015.07.007

Abstract

Abstract:

The study was conducted to investigate the expression characteristics and bioinformatics analysis of beta-defensin family genes in the testes and epididymal caput of bucks.The data were from the transcriptomes of three testes of 7-day-old kids，testes and epididymal caput of three adult bucks of known fertility analyzed by Illumina HiSeq^TM2000 high-throughput RNA sequencing.All beta-defensin family genes were sorted out according to annotation of unigenes and contigs for analysis of different expression.Protein profile of beta-defensins was analyzed by bioinformatics methods.The results showed that a total of 7，12 and 33 β-defensins were discovered in testes of 7-day-old kids，testes and epididymal caput of adult bucks respectively，while gDB123，gDB119 and gDB124 were the highest expression in the testes of 7-day-old kids，testes and epididymal caput of adult bucks respectively.gDB33 was specifically expressed in testes of adult bucks.Twenty-one β-defensins were specifically expressed in epididymal caput of adult bucks.The molecular weight of β-defensins was from 6.89 to 13.85 ku.The bioinformatics analysis showed that goat β-defensins protein were small molecular，polar，hydrophobicity and basic polypeptide with positive charge.A high degrees of conservation in spacing pattern was 6 cysteines in peptides sequence of β-defensins，which consensus pattern was -C-X_2-45-C-X_3-6-C-X_3-13-C-X_4-7-CC-.However，the pattern of 24 β-defensins was -C-X_5，6-C-X_3，4-C-X₉-C-X_5，6-CC-.A total of 16 β-defensins were secreted glycoprotein.Except for gDB126，the rest of 24 β-defensins had the phosphorylation sites.The goat β-defensins family protein were also the antimicrobial cationic peptides.The rich specific-epididymis β-defensins were expressed in epididymal caput，which could coat sperm plasma membrane with glycocalyx in sperm maturation and play a vital role in making sperm motion.And β-defensins maybe were cell signal in some pathways.

CLC Number:

S827.2

ZHANG Chun-xiang，DU Hai-yan，ZHANG Cai-xia，ZHENG Ya-lin，REN You-she，JIANG Yu-suo. Expression Profiles and Bioinformatics Analysis of beta-defensin Family Genes in the Testes and Epididymal Caput of Bucks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1122-1133.

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Expression Profiles and Bioinformatics Analysis of beta-defensin Family Genes in the Testes and Epididymal Caput of Bucks

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